Composite

Part:BBa_K4160010:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


GEMS receptor construct containing PR3 as affinity domain with 8 amino acid linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2363
    Illegal PstI site found at 191
    Illegal PstI site found at 1051
    Illegal PstI site found at 2046
    Illegal PstI site found at 2207
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 733
    Illegal NheI site found at 1420
    Illegal PstI site found at 191
    Illegal PstI site found at 1051
    Illegal PstI site found at 2046
    Illegal PstI site found at 2207
    Illegal NotI site found at 2350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 733
    Illegal BglII site found at 1524
    Illegal BglII site found at 1710
    Illegal BglII site found at 1974
    Illegal BamHI site found at 64
    Illegal XhoI site found at 934
    Illegal XhoI site found at 2357
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2363
    Illegal PstI site found at 191
    Illegal PstI site found at 1051
    Illegal PstI site found at 2046
    Illegal PstI site found at 2207
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2363
    Illegal PstI site found at 191
    Illegal PstI site found at 1051
    Illegal PstI site found at 2046
    Illegal PstI site found at 2207
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002, BBa_K4160004, and BBa_K2217015.

References