Composite
Part:BBa_K4160010:Design
Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel Group: iGEM22_TU-Eindhoven (2022-10-09)
GEMS receptor construct containing PR3 as affinity domain with 8 amino acid linker
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 733
Illegal XbaI site found at 2363
Illegal PstI site found at 191
Illegal PstI site found at 1051
Illegal PstI site found at 2046
Illegal PstI site found at 2207 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 733
Illegal NheI site found at 1420
Illegal PstI site found at 191
Illegal PstI site found at 1051
Illegal PstI site found at 2046
Illegal PstI site found at 2207
Illegal NotI site found at 2350 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 733
Illegal BglII site found at 1524
Illegal BglII site found at 1710
Illegal BglII site found at 1974
Illegal BamHI site found at 64
Illegal XhoI site found at 934
Illegal XhoI site found at 2357 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 733
Illegal XbaI site found at 2363
Illegal PstI site found at 191
Illegal PstI site found at 1051
Illegal PstI site found at 2046
Illegal PstI site found at 2207 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 733
Illegal XbaI site found at 2363
Illegal PstI site found at 191
Illegal PstI site found at 1051
Illegal PstI site found at 2046
Illegal PstI site found at 2207 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002, BBa_K4160004, and BBa_K2217015.